NGS cfDNA Library PrepSet
The product amplifies multiple nucleic acid sequences at the same time through multiple PCR amplification technology, which could achieve high-fidelity PCR amplification of 100,000 to 10,000 pounds. The logarithm of self-customized Panel primers can be between ten and ten thousand pairs. The sequence junction is connected to the amplified target nucleic acid by ligase. The libraries prepared can be used on Ion Torrent o illumina sequencing platforms.
Reagent components included in the product
1. NGS cfDNA Library PrepSet(for Ion Torrent)
Used for the construction of cfDNA library, the constructed gene library can be directly used for high-throughput sequencing on the Ion Torrent platform and to obtain sample nucleic acid sequence information.
2. NGS cfDNA Library PrepSet (for Illumina)
Used for the construction of cfDNA library,,the constructed gene library can be directly used for high-throughput sequencing on the Illumina platform and to obtain sample nucleic acid sequence information.
| High data uniformity |
Uniformity> 95% |
| End primer digestion |
Primer digestion reduces waste of known sequences |
| High library quality in complex areas |
High GC, high mutation, high repetitionand other complex areas are of goodquality |
| Low template start amount |
The starting template can be as low as 1ng |
| Wide range of amplifying enrichment enzyme |
The primer pairs from 10 to 10000 pairs can be effectively amplified to build the library |
Experiment Process
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