Exonuclease III Raw Material Enzyme

• Environmental Protection: Yes
• Classification: Coenzyme
• Transport Package: Carton
• Specification: test
• Trademark: ABIOM
• Origin: China
• Model NO.: Exonuclease III
• HS Code: 3507901000
• Production Capacity: 100000

Product Description

 

Product Description

Catalyzes the stepwise removal of mononucleotides from 3' -hydroxyl termini of duplex DNA , A limited number of nucleotidesare removed during each binding event, resulting in coordinated progressive deletions within the population of DNA moleculesExonuclease ll has also been reported to have RNase H, 3' -phosphatase and AP-endonuclease activities .The preferred substrates are blunt or recessed 3' -termini, although the enzyme also acts at nicks in duplex DNA to producesingle-strand gaps. 3' -protruding termini are resistant to cleavage: the degree of resistance depends on the length of the extension,with extensions 4 bases or longer being essentially resistant to cleavage.
Exonuclease ll activity depends partially on helical structure and displavs seuence dependence (C>A-T>G). Temperature. satconcentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specifitapplications.

 

Product Parameters

Applications

Unidirectional nested deletions;

Site-directed mutagenesis;

Preparation of strand-specific probes;

Preparation of single-stranded substrates for dideoxy sequencing.

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