Basic Info.
Model NO.
Exonuclease Ill
CAS No.
Gsp9901, Gsp9902, Gsp9903, Gsp9904
Classification
Specific Reagents
Specific Usage
For Biological Purpose
Usage
Laboratory Reagents, Diagnostic Reagents
Habit Appellation
Chemical Medicine
Application
Scientific Research, Health
Product Name
Exonuclease Ill
Feature
High Purity, Simple, Efficient
Appearance
Colorless Liquid
Applications
Scientific Research, PCR
Transport Package
Carton Package
Product Description
Product Description
Exonuclease Ill
Catalyzes the stepwise removal of mononucleotides from 3'-hydroxyl termini of duplex DNA. A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules . Exonuclease Ill has also been reported to have RNase H, 3'-phosphatase and AP-endonuclease activities .
The preferred substrates are blunt or recessed 3'-termini, although the enzyme also acts at nicks in duplex DNA to produce single-strand gaps. 3'-protruding termini are resistant to cleavage; the degree of resistance depends on the length of the extension,with extensions 4 bases or longer being essentially resistant to cleavage.
Exonuclease activity depends partially on helical structure and displays sequence dependence (C>A=T>G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications.
Applications | |
1. Unidirectional nested deletions | |
2. Site-directed mutagenesis | |
3. Preparation of strand-specific probes |
4.Preparation of single-stranded substrates for dideoxy sequencing Peformance |
Address:
C1building, No. 11 Kaiyuan Road Science City, High-Tech Industrial Development District, Guangzhou, Guangdong, China
Business Type:
Manufacturer/Factory
Business Range:
Health & Medicine, Instruments & Meters
Company Introduction:
GuangDong Ardent Biomed Co., Ltd is an American medical technology company, initially founded as a product development and research lab in Silicon Valley California in 2011. In 2016 ABIOM established its Product manufacturing and Marketing Center and genetic testing service center in Guangzhou High-Tech Industrial Development Zone, With strong R&D team, ABIOM has developed over 120 products and owns 100 patents, including more than 20 authorized invention patents and two PCT invention patents.
With the mission of "Health Brings Prosperity", ABIOM focuses on Precision medicine and is committed to becoming an innovation leader on accurate diagnosis in immune and infectious diseases that accounts for 66% of the trillion IVD market.
Our vision is to "ABIOM improves health" Ardent has developed the world′s first leading Microfluidic HDL Subtyping System(with instrument and reagent kits), which improves the accurate detection and risk assessment of the world′s number one killer cardiovascular disease(CVD) at a very early period(before the age of 45).
ABIOM genetic testing service center covers its testing services with four categories: Immune and Inflammatory Diseases, Infectious Diseases, Reproductive Health, and Cancer.
Guangdong Ardent Biomed Co., Ltd is one of the very few high-tech IVD enterprises in China that provides a one-stop service from R&D to production of raw materials (enzyme etc. ), reagent kits, medical consumables, instruments, as well as molecular diagnostic services, expert interpretation and health management services.
The company founder, Dr. David Deng, has more than 20-year experience of leading diagnostic product R& AMP; D in Silicon Valleys of three world-known public companies: Agilent Tech, Varinata, Illumina and Ion Torrent, Thermofisher.
Our main business:
Whole range of Nucleic Acid Extraction kit, disposal sampling kit, Nucleic Acid Detection Kit etc.
Reagent kits, devices
Molecular diagnostic testing services
Research services