Molecular Reagents Exonuclease Enzyme

• Customization: Available
• CAS No.: Gsp9901, Gsp9902, Gsp9903, Gsp9904
• Formula: N/a
• Model NO.: Exonuclease Ill
• EINECS: N/a
• Classification: Specific Reagents
• Grade: N/a
• Specific Usage: For Biological Purpose
• Content: N/a
• Usage: Laboratory Reagents, Diagnostic Reagents
• Source: N/a
• Habit Appellation: Chemical Medicine
• Application: Scientific Research, Health
• Property: Organic Reagent
• Product Name: Exonuclease Ill
• Feature: High Purity, Simple, Efficient
• Storage: -25°c-15°c
• Appearance: Colorless Liquid
• Applications: Scientific Research, PCR
• Shelf Life: 18 Months
• Payment: Tt
• Delivery: 5-15 Days
• Transport Package: Carton Package
• Trademark: ABIOM
• Origin: Guangdong, China
• HS Code: 3822190090
• Production Capacity: 5000 Boxes/Months

Product Description

Product Description

Exonuclease Ill

      Catalyzes the stepwise removal of mononucleotides from 3'-hydroxyl termini of duplex DNA. A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules . Exonuclease Ill has also been reported to have RNase H, 3'-phosphatase and AP-endonuclease activities . 
      The preferred substrates are blunt or recessed 3'-termini, although the enzyme also acts at nicks in duplex DNA to produce single-strand gaps. 3'-protruding termini are resistant to cleavage; the degree of resistance depends on the length of the exten­sion,with extensions 4 bases or longer being essentially resistant to cleavage. 
     Exonuclease activity depends partially on helical structure and displays sequence dependence (C>A=T>G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications. 

Applications
1. Unidirectional nested deletions
2. Site-directed mutagenesis
3. Preparation of strand-specific probes
4.Preparation of single-stranded substrates for dideoxy sequencing Peformance