Crispr Aapcas12b Lbcas12A Lbucas13A Lwacas13A

• Environmental Protection: Yes
• Classification: Coenzyme
• Transport Package: Carton
• Specification: test
• Trademark: ABIOM
• Origin: China
• Model NO.: CRISPR
• HS Code: 3507901000
• Production Capacity: 100000

Product Description

 

Product Description

Also known as C2cl, purified from E. coli strain containing the Alicyclobacillus acidophilus Cas12b expression gene. lt belongs tothe V-type CRISPR Cas system. ln the presence of PAM in the target DNA, it is a RNA mediated Nuclease that specifically targets to cuidouble stranded DNA. The binding of LbCas12b crRNA complex with complementary single stranded DNA or double stranded DNAreleases strong non-specific DNA transcleavage activity. Compared to Cas13a, Cas12b can maintain higher activity under loopmediated isothermal amplification (LAMP) reaction conditions.

LbCas12a was purified from E. coli strain containing the Lachnospiraceae bacterium CRISPR-Cas12a expression gene. LbCas12abelongs to V-type CRISPR Cas system, n the presence of PAM in the target DNA, it is a RNA mediated Nuclease that specifically targetsto cut double stranded DNA. Compared to Cas9, Cas12a only requires a single crRNA and has a smaller volume, making it easier todeliver into cells: At the same time. the combination of lbCas12a crRNA complex with complementary single stranded DNA or doublestranded DNA releases strong non-specific SSDNA trans cleavage activity, which can be used for Genetic testing and imaging.

LwaCas13a was purified from E. coli strain containing the Leptotrichia wadei CRISPR-Cas13a (C2c2) expression gene. LwaCas13ais a type Vl CRISPR Cas system, which is an RNA mediated Nuclease targeting RNA cleavage. LwaCas13a has high specificity andsensitivity, and can be used to detect individual RNA molecules. MIT Zhang Feng team has thus established the "SHERLOCK" molecular diagnosis system, which has broad application prospects in the field of Nucleic acid test.

LbuCas13a was purified from E. coli strain containing the exression gene of leptotrichia buccalis CRISPR-Cas13a. LbuCas13a is 2type Vl CRISPR Cas system, which is a RNA mediated Nuclease targeting RNA cleavage. LbuCas13a has high specificity and sensitivityand can be used to detect individual RNA molecules. The Nobel Prize winner Doudna team has established a tandem CRISPR Nuclase system based on this, which has a broad application prospect in the field of Nucleic acid test.

 

Product Parameters

Applications

Multiple enzymes work together at room temperature to achieve rapid amplification of nucleic acids, which is a truePOCT technology. lt is a next-generation high sensitivity, high specificity, fast isothermal Nucleic acid test technologybased on CRISPR detection development.

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