Customization: | Available |
---|---|
Product Name: | Endonuclease IV (Nfo) |
Feature: | Hight Purity |
Still deciding? Get samples of US$ 20/Piece
Request Sample
|
Suppliers with verified business licenses
Audited by an independent third-party inspection agency
Endonuclease IV (Nfo)(10 U/μL)
This product originates from E.coli and is involved in DNA damage repair. This enzyme can recognize the depurine/depyrimidine (AP) sites on double stranded DNA molecules and cleave the first phosphate diester bond at the 5' end of the AP site, producing 3'
hydroxyl and 5' deoxyribose phosphate terminals; In addition, endonuclease IV also has 3' diesterase activity, which can release
phosphoglyceraldehyde, intact deoxyribose 5'- phosphate, and phosphate from the 3 'end of DNA. This enzyme also has 3'-5 'exonuclease activity, and its progressive action on the substrate is influenced by metal ions, DTT, EDTA, etc., and is more inclined to act on double stranded DNA at the 3' concave end.
Applications:
1. Single cell gel electrophoresis; |
2. Research on DNA damage and repair; |
3. SN P analysis. |