Crispr Enzymes Aapcas12b Lbcas12A Lwacas13A Lbucas13A

• CAS No.: Gsp9291, Gsp9292, Gsp9281, Gsp9282, Gsp9311, Gsp9312
• Formula: N/a
• EINECS: N/a
• Classification: Biochemical Reagents
• Grade: N/a
• Specific Usage: For Biological Purpose
• Model NO.: AapCas12b, LbCas12a, LwaCas13a, LbuCas13a
• Content: Standard
• Usage: Laboratory Reagents
• Source: /
• Habit Appellation: /
• Application: Scientific Research
• Property: Enzyme & Coenzyme
• Product Name: Crispr
• Feature: High Purity, Simple, Efficient
• Storage: -25°c-15°c
• Appearance: Colorless Liquid
• Applications: Scientific Research, PCR
• Shelf Life: 18 Months
• Payment: Tt
• Delivery: 5-15 Days
• Packing: Box
• Shipping: Express, by Air
• Transport Package: Carton Package
• Specification: 20µ L, 100µ L, 2mL
• Trademark: ABIOM
• Origin: Guangdong, China
• Production Capacity: 5000 Boxes/Month

Product Description

Product Description

 

AapCas12b

Also known as C2cl, purified from E.coli strain containing the Alicyclobacillus acidophilus Cas12b expression gene. It belongs to
the V-type CRISPR Cas system. In the presence of PAM in the target DNA, it is a RNA mediated Nuclease that specifically targets to cut
double stranded DNA. The binding of LbCas12b crRNA complex with complementary single stranded DNA or double stranded DNA
releases strong non-specific ssDNA transcleavage activity. Compared to Casl3a, Cas12b can maintain higher activity under loop
mediated isothermal amplification (LAMP) reaction conditions.

LbCas12a
LbCas12a was purified from E.coli strain containing the Lachnospiraceae bacterium CRISPR-Cas12a expression gene. LbCas12a belongs to V-type CRISPR Cas system. In the presence of PAM in the target DNA, it is a RNA mediated Nuclease that specifically targets to cut double stranded DNA. Compared to Cas9, Cas12a only requires a single crRNA and has a smaller volume, making it easier to deliver into cells; At the same time, the combination of LbCas12a crRNA complex with complementary single stranded DNA or double stranded DNA releases strong non-specific ssDNA trans cleavage activity, which can be used for Genetic testing and imaging.

 

LwaCas13a
LwaCas13a was purified from E.coli strain containing the Leptotrichia wadei CRISPR-Cas13a (C2c2) expression gene. LwaCas13a is a type VI CRISPR Cas system, which is an RNA mediated Nuclease targeting RNA cleavage. LwaCas13a has high specificity and sensitivity, and can be used to detect individual RNA molecules. MIT Zhang Feng team has thus established the "SHERLOCK" molecular diagnosis system, which has broad application prospects in the field of Nucleic acid test.

LbuCas13a

LbuCas13a was purified from E.coli strain containing the expression gene of Leptotrichia buccalis CRISPR-Cas13a. LbuCas13a is a
type VI CRISPR Cas system, which is a RNA mediated Nuclease targeting RNA cleavage. LbuCas13a has high specificity and sensitivity, and can be used to detect individual RNA molecules. The Nobel Prize winner Doudna team has established a tandem CRISPR Nuclease system based on this, which has a broad application prospect in the field of Nucleic acid test.

Applications: 
 

Multiple enzymes work together at room temperature to achieve rapid amplification of nucleic acids, which is a true POCT technology. It is a next-generation high sensitivity, high specificity, fast isothermal Nucleic acid test technology based on CRISPR detection development.

Performance: